Journal: Journal of medicinal chemistry

Article Title: A novel N-substituted valine derivative with unique PPARγ binding properties and biological activities

doi: 10.1021/acs.jmedchem.0c01555

Figure Lengend Snippet: Effect of 7j on PPARγ phosphorylation. (A) Percentage of in vitro PPARγ Ser273 phosphorylation by CDK5 in the presence of 0.1 μM of 7j or Rosi. (B) Phosphorylation of PPARγ Ser273 in 3T3-L1 adipocyte incubated for 60 minutes with 5 μM of Rosi, 7j or Roscovitine (Rsv) before TNFα stimulation (50 ng/ml; 90 minutes). Two independent experiments are shown. Phosphospecific antibodies were from Rockland or New England Peptide (NEP). Arrow heads indicate phosphorylated PPARγ and PPARγ2. (C) RT-PCR analysis of the expression levels of a selection of genes known to be regulated by CDK5-dependent phosphorylation of PPARγ. Values are means ± SD (n = 5) expressed relative to the mean of control. **p < 0.01, ***p < 0.001 vs. control (one-way ANOVA followed by Dunnett’s post hoc test).

Article Snippet: PPARγ phospho Ser273 antibodies were from Rockland (Limerick, PA, USA) or custom produced by New England Peptide (Gardner, MA, USA).

Techniques: Phospho-proteomics, In Vitro, Incubation, Reverse Transcription Polymerase Chain Reaction, Expressing, Selection, Control

Journal: Research

Article Title: Paeoniflorin Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by SYK/SH3BP2 Signaling Pathway

doi: 10.34133/research.1100

Figure Lengend Snippet: PF alleviated lipid metabolism disorders induced by HFFD in MASLD mice. (A and B) Hepatic TG and TC levels. (C and D) ORO staining and quantitative results ( n = 3, scale bar = 50 μm). (E) mRNA levels in liver. (F and G) Western blot detection of hepatic SCD1 and PPAR-γ protein expression. Data were presented as the mean ± SEM ( n = 6). ## P < 0.01, ### P < 0.001 versus the ND group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the HFFD group.

Article Snippet: The antibodies used are as follows: SCD1 (2794T, CST, 1:1,000), PPAR-γ (66936-1-Ig, Proteintech, 1:2,000), IL-6 (12242T, CST, 1:1,000), TLR4 (66350-1-Ig, Proteintech, 1:3,000), TIMP1 (33502-1, SAB, 1:3,000), α-SMA (67735, Proteintech , 1:20,000), Tgf-β1 (26155, Proteintech, 1:1,000), α-tubulin (66031, Proteintech, 1:20,000), GAPDH (60004-1-IG, Proteintech, 1:6,000), Phospho-Syk (2710T, CST, 1:1,000), Syk (2712T, CST, 1:1,000), SH3BP2 (PA5-87739, Invitrogen, 1:1,000), Goat anti-Mouse IgG Secondary Antibody HRP (L3032, SAB, 1:5,000), and Goat anti-Rabbit IgG Secondary Antibody HRP (L3012, SAB, 1:5,000).

Techniques: Staining, Western Blot, Expressing

Journal: Research

Article Title: Paeoniflorin Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by SYK/SH3BP2 Signaling Pathway

doi: 10.34133/research.1100

Figure Lengend Snippet: PF ameliorated dysregulated hepatic lipid metabolism and inflammation in MASLD via SYK/SH3BP2 pathway inhibition in vitro. (A) LO2 cell viability ( n = 3). (B and C) LO2 cell TG and TC content ( n = 3). (D and E) Representative graphs and quantification of LO2 cell ORO staining ( n = 3, scale bar = 200 μm). (F to L) Western blot of P-SYK, SYK, SH3BP2, TLR4, IL-6, PPAR-γ, and SCD1 protein expression and their quantification. (M) SYK, SH3BP2, ACC, SREBP-1C, SCD1, PPAR-γ, CD36, CPT1α, PPAR-α, ATGL, FASN, CCR2, CD44, TLR4, IL-6, IL-1β, TNF-α, MCP-1 , and IL-6 mRNA levels. Data were presented as the mean ± SEM ( n = 4). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the PO group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the PO+oe-SYK group.

Article Snippet: The antibodies used are as follows: SCD1 (2794T, CST, 1:1,000), PPAR-γ (66936-1-Ig, Proteintech, 1:2,000), IL-6 (12242T, CST, 1:1,000), TLR4 (66350-1-Ig, Proteintech, 1:3,000), TIMP1 (33502-1, SAB, 1:3,000), α-SMA (67735, Proteintech , 1:20,000), Tgf-β1 (26155, Proteintech, 1:1,000), α-tubulin (66031, Proteintech, 1:20,000), GAPDH (60004-1-IG, Proteintech, 1:6,000), Phospho-Syk (2710T, CST, 1:1,000), Syk (2712T, CST, 1:1,000), SH3BP2 (PA5-87739, Invitrogen, 1:1,000), Goat anti-Mouse IgG Secondary Antibody HRP (L3032, SAB, 1:5,000), and Goat anti-Rabbit IgG Secondary Antibody HRP (L3012, SAB, 1:5,000).

Techniques: Inhibition, In Vitro, Staining, Western Blot, Expressing

Journal: PLoS ONE

Article Title: Expression of Obesity Markers and Persistent Organic Pollutants Levels in Adipose Tissue of Obese Patients: Reinforcing the Obesogen Hypothesis?

doi: 10.1371/journal.pone.0084816

Figure Lengend Snippet: Data represent mean (±SE) of the serum concentrations of leptin and adiponectin (A) and gene expression of leptin, adiponectin, TNFα and PPARγ normalised to the gene expression of TBP gene*1000 of AT samples (B, C, D, E). Significant differences between men (n = 17) and women (n = 33) (B, C), diabetic (n = 8) and non diabetic patients (n = 42) (D, E) are indicated with asterisks (Mann Whitney U-test; *p≤0.05; **p≤0.01).

Article Snippet: Template standards and primers were obtained from OriGene Technologies (Rockville, MD) for every measured gene: PPARγ (HK210365), TNFα (HK208349), ADIPOQ (HK209573) and LEP (HK204316).

Techniques: Expressing, MANN-WHITNEY

Journal: eLife

Article Title: Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferation

doi: 10.7554/eLife.30862

Figure Lengend Snippet:

Article Snippet: recombinant DNA reagent , pCMV6-XL4 PPARG (plasmid) , OriGene , OriGene:SC108192 , .

Techniques: Generated, Plasmid Preparation, Infection, In Vitro, Recombinant, Mutagenesis, Clone Assay, Sequencing, Luciferase, Software